Comprehensive STD and STI testing options available at a convenient Middlesboro location, emphasizing privacy, speed, and medically accurate lab testing.
Our team is standing by to answer your questions and schedule your confidential STD testing appointment today.
Comprehensive STD and STI testing options available at a convenient Middlesboro location, emphasizing privacy, speed, and medically accurate lab testing.

Our most comprehensive panel includes HIV 1&2, Syphilis, Gonorrhea, Chlamydia, Hepatitis A/B/C, and Herpes 1&2. Ideal for routine sexual health checkups, this FDA-approved, CLIA-processed panel provides peace of mind and full coverage available at our Middlesboro center.
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Test for specific concerns like HIV, Herpes, Chlamydia, Gonorrhea, Syphilis, or Hepatitis. Cost-effective and quick, individual tests are perfect for targeted screening based on exposure, symptoms, or clinician recommendation with easy phone ordering.
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We offer 4th generation HIV antibody/p24 antigen testing and FDA-cleared HIV RNA early detection for the earliest reliable results (as early as 10-12 days post-exposure). Laboratory-based testing ensures accuracy and confidential counseling with results delivered securely.
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Type-specific IgG blood testing distinguishes HSV-1 and HSV-2, important for asymptomatic people and those with concerns. Tests offer accurate clinical interpretation and are processed in CLIA-certified labs, available at our Middlesboro location.
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Chlamydia and gonorrhea are common and often asymptomatic. We use urine-based NAAT testing when appropriate for simple, accurate detection. Quick turnaround and treatment support available if needed at local clinics.
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Comprehensive hepatitis panels cover A, B, and C with blood-based testing. Early detection prevents liver complications and is recommended for higher-risk individuals. Hepatitis testing is included in many panels and processed at CLIA-certified partner labs.
Learn more →Call for a confidential consultation and same-day appointments in Middlesboro. Our knowledgeable, compassionate staff helps you order tests by phone for privacy and provides supportive, non-judgmental care.
Same-day appointments available
📞 Call Now for Confidential TestingRegular STD testing protects your health and the well-being of partners. Many STIs are asymptomatic, so you may not know you are infected — the CDC estimates about 1 in 5 Americans has an STI. In Southeast Kentucky, including Middlesboro and surrounding communities near Cumberland Gap, routine screening is especially important for sexually active adults, people with new or multiple partners, pregnant people, and anyone with symptoms. The CDC recommends at least annual testing for sexually active individuals and more frequent screening for higher-risk groups. Early detection allows for timely treatment, prevents complications like infertility or liver disease, and reduces the risk of transmission. Testing is a routine part of responsible sexual health; normalizing screening reduces stigma and empowers you to make informed decisions. Our Middlesboro services provide confidential STI screening, rapid laboratory turnaround, and compassionate counseling so you can get answers and next steps quickly and privately.